doc. Jose Luis Ros-Santaella, Ph.D.



3. Pintus E, Ros-Santaella JL (2021). Laboratory Protocols in Animal Physiology. Part II: Clinical Biochemistry. Published by Czech University of Life Sciences Prague. 65 pp. ISBN: 978-80-213-3090-0.

2. Pintus E, Ros-Santaella JL (2020). Laboratory Protocols in Animal Physiology. Part I: Blood Analysis in Mammals. Published by Czech University of Life Sciences Prague. 66 pp. ISBN: 978-80-213-3023-8.

1. Moreno-Rueda G, Casas F, Collantes-Martín E, Comas M, Megía-Palma R, Ochoa D, Pintus E, Pizarro M, Reguera S, Rivas JM, Ros-Santaella JL & Zamora-Camacho FJ (editors). International Congress of the Spanish Society of Ethology and Evolutionary Ecology (Book of Abstracts). Edited by: Spanish Society of Ethology and Evolutionary Ecology and University of Granada (2016).157 pp. ISBN: 978-84-617-4865-5.



1. Ros-Santaella JL, Munilla-León T (2005). Picnogónidos o arañas de mar. Generalidades y Fauna Andaluza. En: Proyecto Andalucía. Naturaleza. Tomo XVI. Zoología IV. Capítulo 15 (Ed: Publicaciones comunitarias, S.L. –Grupo Hércules-), pp 284-298. ISBN: 84-933178-8-8. In Spanish.


PAPERS (SCI journals)

34. Garrido-Bautista J, Hernández-Ruiz C, Ros-Santaella JL, Pintus E, Bernardo N, Comas M, Moreno-Rueda G. Habitat-dependent breeding biology of the Blue Tit (Cyanistes caeruleus) across a continuous and heterogeneous Mediterranean Woodland (2023). Avian research, in press.

33. Pintus E, Chinn AF, Kadlec M, García-Vázquez FA, Novy P, Matson JB, Ros-Santaella JL*. N-thiocarboxyanhydrides, amino acid-derived enzyme-activated H2S donors, enhance sperm mitochondrial activity in presence and absence of oxidative stress (2023). BMC Veterinary Research, 19:52.

32. Moreno-Rueda G, Garrido-Bautista J, Soria A, Trenzado CE, Pérez-Jiménez A, Pintus E, Ros-Santaella JL, Bernardo N, Comas M, Kolenčík S. Within-brood body size and immunological differences in blue tit (Cyanistes caeruleus) nestlings relative to ectoparasitism (2022). Avian research, (13):100038.

31. Garrido-Bautista J, Martínez-de la Puente J, Ros-Santaella JL, Pintus E, Lopezosa P, Bernardo N, Comas M, Moreno-Rueda G. Habitat-dependent Culicoides species composition and abundance in blue tit (Cyanistes caeruleus) nests (2022). Parasitolgy, 149(8):1119-1128.    

30. Kadlec M, Pintus E, Ros-Santaella JL. The Interaction of NO and H2S in Boar Spermatozoa under Oxidative Stress (2022). Animals, 12, 602.

29. Muñoz-Baquero M, Marco-Jiménez F, García-Dominguez X, Ros-Santaella JL, Pintus E, Jiménez-Movilla M, García-Párraga D, García-Vázquez FA. Comparative study of semen parameters and hormone profile in small-spotted catshark (Scyliorhinus canicula): aquarium housed vs wild-captured (2021). Animals, 11(10), 2884.

28. Pintus E, Ros-Santaella JL. Impact of Oxidative Stress on Male Reproduction in Domestic and Wild Animals (2021). Antioxidants, 10, 1154.

27. Fuentes-Albero MC, González Brusi L, Cots P, Luongo C, Abril Sánchez S, Ros Santaella JL, Pintus E, Ruiz Diaz S, Barros García C, Sánchez Calabuig MJ, García-Párraga D, Avilés M, Izquierdo Rico MJ, García Vázquez FA. Protein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus) (2021). Frontiers in Cell and Developmental Biology, 9:673961.

26. Garrido-Bautista J, Soria A, Trenzado CE, Pérez-Jiménez A, Ros-Santaella JL, Pintus E, Bernardo N, Comas M, Moreno-Rueda G. Oxidative status of blue tit nestlings varies with habitat and nestling size (2021). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 258, 110986.

25. Ros-Santaella JL*, Pintus E (2021). Plant Extracts as Alternative Additives for Sperm Preservation. Antioxidants, 10(5), 772.

24. Fuentes-Albero MC, Abril-Sánchez S, Ros-Santaella JL, Pintus E, Luongo C, Ruiz-Díaz S, Barros-García C, Sánchez-Calabuig MJ, García-Párraga D, García-Vázquez FA (2021). Characterization of Bottlenose Dolphin (Tursiops truncatus) Sperm Based on Morphometric Traits. Biology, 10(5), 355.

23. Pintus E, Kadlec M, Karlasová B, Popelka M, Ros-Santaella JL* (2021). Spermatogenic Activity and Sperm Traits in Post-Pubertal and Adult Tomcats (Felis catus): Implication of Intra-Male Variation in Sperm Size. Cells, 10(3), 624.

22. Pintus E, Jovičić M, Kadlec M, Ros-Santaella JL* (2020). Divergent effect of fast- and slow-releasing H2S donors on boar spermatozoa under oxidative stress. Scientific Reports10, 6508.

21. Kadlec M, Ros-Santaella JL, Pintus E (2020). The Roles of NO and H2S in Sperm Biology: Recent Advances and New Perspectives. International Journal of Molecular Sciences, 21(6), 2174.

20. Ros-Santaella JL*, Kadlec M, Pintus E (2020). Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress. Animals, 10(3), 463.

19. Ruiz-Díaz S, Luongo C, Fuentes-Albero MC, Abril-Sánchez S, Sánchez-Calabuig MJ, Barros-García C, De la Fe C, García-Galán A, Ros-Santaella JL, Pintus F, Garcia-Párraga D, García-Vázquez FA (2020). Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration. Animal Reproduction Science, 212:106248.

18. Ros-Santaella JL*, Kotrba R, Pintus E (2019). High-energy diet enhances spermatogenic function and increases sperm midpiece length in fallow deer (Dama dama) yearlings. Royal Society Open Science, 6: 181972.

17. Pintus E, Kadlec M, Jovičić M, Sedmíková M, Ros-Santaella JL* (2018). Aminoguanidine Protects Boar Spermatozoa Against the Deleterious Effects of Oxidative Stress. Pharmaceutics, 10(4), 212.

16. Jovičič M, Pintus E, Fenclová T, Šimoník O, Chmelíková E, Ros-Santaella JL, Sedmíková M (2018). Effect of nitric oxide on boar sperm motility, membrane integrity, and acrosomal status during semen storage. Polish Journal of Veterinary Sciences, 21(1): 73-82.

15. Pintus E, Ros-Santaella JL* (2018). Assessment of reproductive traits in male gemsbok (Oryx gazella). Spixiana, 41(2): 206-209.

14. Zahumenická P, Fernández E, Šedivá J, Žiarovská J, Ros‑Santaella JL, Martínez‑Fernández D, Russo D, Milella L (2018). Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L. Plant Cell, Tissue and Organ Culture, 132(2): 317-327.

13. Ros-Santaella JL*, Pintus E (2017). Rooibos (Aspalathus linearis) extract enhances boar sperm velocity up to 96 hours of semen storage. PLoS ONE 12(8):e0183682.

12. Grieblová A, Pintus E, Ros-Santaella JL* (2017). Integrity of head and tail plasmalemma is associated with different kinetic variables in boar sperm. Animal Reproduction Science, 184: 218–227.

11. Ros-Santaella JL*, Pintus E, Garde JJ (2015). Intramale variation in sperm size: functional significance in a polygynous mammal. PeerJ 3:e1478.

10. Pintus E, Ros-Santaella JL*, Garde JJ (2015). Beyond testis size: links between spermatogenesis and sperm traits in a seasonal breeding mammal. PLoS ONE 10(10):e0139240.

9. Ros-Santaella JL*, Pintus E, Senhadji K (2015). Cannibalistic behaviour of the vulnerable and endemic Baetica ustulata (Rambur, 1838) (Orthoptera: Tettigoniidae: Ephippigerinae): first report. Annales de la Société entomologique de France 51(2): 89–92.

8. Pintus E, Ros-Santaella JL, Garde JJ (2015). Variation of spermatogenic and Sertoli cell number detected by Fine Needle Aspiration Cytology (FNAC) in Iberian red deer during and out of breeding season. Reproduction, Fertility and Development 27(5): 812–822.

7. Ros-Santaella JL*, Domínguez-Rebolledo AE, Garde JJ (2014). Sperm flagellum volume determines freezability in red deer spermatozoa. PLoS ONE 9(11):e112382.

6. Pintus E, Ros-Santaella JL, Garde JJ (2014). Diagnostic value of Fine Needle Aspiration Cytology (FNAC) in testicular disorders of red deer: a case report. Journal of Wildlife Diseases 50(4): 994–997.  

5. Vidal-Cordero M, Moreno-Rueda G, López-Orta A, Marfil-Daza C, Ros-Santaella JL, Ortiz-Sánchez J (2012). Brighter-colored paper wasps (Polistes dominula) have larger poison glands. Frontiers in Zoology 9:20.

4. MR Fernández-Santos, AJ Soler, M. Ramón, JL Ros-Santaella, A Maroto-Morales, O García-Álvarez, A Bisbal, JJ Garde, MA Coloma, J. Santiago-Moreno (2011). Effect of post-mortem time on post-thaw characteristics of Spanish ibex (Capra pyrenaica) spermatozoa. Animal Reproduction Science 129(1-2):56–66.

3. Domínguez-Rebolledo AE, Martínez-Pastor F, Bisbal AF, Ros-Santaella JL, García-Álvarez O, Maroto Morales A, Soler AJ, Garde JJ, Fernández-Santos MR (2011). Response of thawed epididymal red deer spermatozoa to increasing concentrations of hydrogen peroxide, and importance of individual male variability. Reproduction in Domestic Animals 46(3):293–303.

2. Domínguez-Rebolledo AE, Fernández-Santos MR, Bisbal AF, Ros-Santaella JL, Ramón M, Carmona M, Martínez-Pastor F, Garde JJ (2010). Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reproduction, Fertility and Development 22(5):856–870.

1. Domínguez-Rebolledo AE, Martínez-Pastor F, Fernández-Santos MR, del Olmo E, Bisbal AF, Ros-Santaella JL, Garde JJ (2010). Comparison of the TBARS and BODIPY C11 probes for assessing lipid peroxidation in red deer spermatozoa. Reproduction in Domestic Animals 45(6):360–368.

*corresponding author


PAPERS (NO SCI journals)

9. Ros-Santaella JL, Pintus E, Chmelíková E, Sedmíková M. (2021). Vliv abnormalit spermií na reprodukčni potenciál kanců. Naš chov 3, 30-31.

8. Pintus E., Ros-Santaella JL, Chmelíková E., Sedmíková M. (2021). Dopad globálního oteplování na reprodukci hospodářských zvířat. Naš chov 1, 14-15

7. Pintus E, Ros-Santaella JL (2019). L'incredibile biodiversità degli spermatozoi. Sapere 49: 30-35.

6. Pintus E, Ros-Santaella JL, Kadlec M. (2019). Neuvěřitelná rozmanitost spermií v živočišné říši. Živá Příroda 6: 42-45.

5. Pintus E, Ros-Santaella JL (2018). Radicali liberi, antiossidanti e fertilità maschile. Micron.  Available at: www.rivistamicron.it

4. Ros-Santaella JL, Pintus E (2017). Uso del extracto de rooibos (Aspalathus linearis) para la conservación del semen porcino. Suis, 143: 14–18.

3. Pintus E, Ros-Santaella JL (2014). Assisted Reproductive Technologies in deer (Artiodactyla, Cervidae): state of the art. Scientia Agriculturae Bohemica, 45(2):136–146.

2. Tinaut A, Ruano F, Ros-Santaella JL, Senhadji K, Pascual F (2008). Sierra Nevada. La mayor biodiversidad de Iberia. Nova Ciencia, 34: 22–25. 

1. Ros-Santaella, JL (2004). Pycnogonids (Arthropoda, Pycnogonida) from Granada litoral: faunistic, ecological distribution and phenological data. Acta Granatense, 3: 3–10. 




2. Vidal-Cordero JM, Moreno-Rueda G, López-Orta A, Marfil-Daza C, Ros-Santaella JL, Ortiz-Sánchez FJ. Las avispas Polistes dominula más brillantes tienen glándulas de veneno más grandes. XIV National Conference and XI Ibero-American of Ethology. Sevilla (Spain), 11-15 Sep 2012.

1. Pascual F, Senhadji K, Ros-Santaella JL, Ruano F y Tinaut JA. Cartografiado y propuestas para la gestión de varias especies singulares de insectos en el Parque Nacional de Sierra Nevada. XXV Jornadas de la Asociación Española de Entomología. Málaga (Spain), 3-6 Sep 2007.


10. Garrido-Bautista J, Soria A, Trenzado CE, Pintus E, Ros-Santaella JL, Bernardo N, Peréz-Jiménez A, Comas M, Moreno-Rueda G. Is hatching asynchrony an effective defence mechanism to ectoparasitism in altricial birds? A test of the Tasty Chick Hypothesis in blue tits. VII biennial congress of SESBE (Spanish Society of Evolutionary Biology). Seville (Spain), 5-7 Feb 2020.

9. Pintus E, Kotrba R, Ros-Santaella JL. El tamaño de los cuernos como indicador del desarrollo sexual en el eland común (Taurotragus oryx).  International Congress of the Spanish Society of Ethology and Evolutionary Ecology, Granada (España), 20-23 Sep 2016.

8. Pintus E., Ros-Santaella J.L., Garde J.J. Features of spermatogenesis in deer with testicular and epididymal disorders. International Conference on Diseases of Zoo and Wild Animals, Barcelona (España), 13-16 May 2015.

7. Hrdlicková M, Fernández Cusimamani E, Ziarovská J, Ros-Santaella JL, Holík A. Assessment of Genetic Fidelity of Micropropagated Plants and in vitro Polyploidisation in Monarda didyma. Tropentag, Prague, Czech Republic, 17-19 Sep 2014.

6. Ros-Santaella, J.L., Pintus, E., Garde J.J. High testicular size is related with low intramale variation of sperm design in red deer. World Congress of Reproducitive Biology, Edinburgh, Scotland, 2-4 Sep 2014.

5. Pintus, E., Ros-Santaella, J.L., Kotrba, R. Spermatogenesis in pubertal common eland (Taurotragus oryx, Pallas 1766). World Congress of Reproductive Biology, Edinburgh, Scotland, 2-4 Sep 2014.

4. Ros-Santaella, J.L., Pintus, E., Kotrba, R., Rajmon, R. Sperm morphometry in the common eland (Taurotragus oryx): preliminary results. Zoologické dny, Ostrava, Czech Republic, 6-7 Jan 2014.

3. Pintus E, Ros-Santaella JL, Bisbal A, Del Olmo E, Dominiguez-Rebolledo A, Fernandez-Santos MR, Soler AJ, Garde José J, Ledda S. Fine needle aspiration cytology (FNAC) as a useful tool to investigate spermatogenesis in Iberian red deer. 8th International Conference on Behaviour, Physiology and Genetics of Wildlife (IZW Leibniz Institute for Zoo and Wildlife Research). Berlin (Germany), 14-17 Sep 2011.

2. Domínguez-Rebolledo AE, Martínez-Pastor F, Fernández-Santos MR, del Olmo E, Esteso MC, Ros-Santaella JL, Bisbal AF, Soler AJ, Garde JJ. Comparison of three extenders for the cryopreservation of red deer (Cervus elaphus hispanicus) epididymal spermatozoa. The European Society for Domestic Animal Reproduction (ESDAR). Belgium, 9-12 Oct 2009.

1. Martínez-Pastor F, Fernández-Santos MR, Domínguez-Rebolledo AE, Esteso MC, Ros-Santaella JL, Bisbal AF, Soler AJ, Garde JJ. Washing increases the vulnerability of red deer thawed spermatozoa to oxidative stress. Reproduction, Fertility and Development. San Diego, California, USA, 3-7 Jan 2009.